Primary Identifier | MGI:6285760 | Allele Type | Targeted |
Attribute String | Null/knockout, Recombinase, Reporter | Gene | Grp |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
Is Recombinase | true | Is Wild Type | false |
molecularNote | The targeting vector was designed to insert an enhanced green fluorescent protein sequence (EGFP) fused to a Cre Recombinase sequence (cre) followed by a FRT-flanked neomycin cassette into the exon 1 ATG start codon of the gene. Flp-mediated recombination removed the FRT-flanked neo cassette. Immunohistochemistry and RNA in situ hybridization confirms the absence of Grp. |