| First Author | Ying G | Year | 2019 |
| Journal | J Biol Chem | Volume | 294 |
| Issue | 11 | Pages | 3957-3973 |
| PubMed ID | 30647131 | Mgi Jnum | J:275959 |
| Mgi Id | MGI:6307339 | Doi | 10.1074/jbc.RA118.006371 |
| Citation | Ying G, et al. (2019) Deletion of both centrin 2 (CETN2) and CETN3 destabilizes the distal connecting cilium of mouse photoreceptors. J Biol Chem 294(11):3957-3973 |
| abstractText | Centrins (CETN1-4) are ubiquitous and conserved EF-hand-family Ca(2+)-binding proteins associated with the centrosome, basal body, and transition zone. Deletion of CETN1 or CETN2 in mice causes male infertility or dysosmia, respectively, without affecting photoreceptor function. However, it remains unclear to what extent centrins are redundant with each other in photoreceptors. Here, to explore centrin redundancy, we generated Cetn3 (GT/GT) single-knockout and Cetn2 (-/-);Cetn3 (GT/GT) double-knockout mice. Whereas the Cetn3 deletion alone did not affect photoreceptor function, simultaneous ablation of Cetn2 and Cetn3 resulted in attenuated scotopic and photopic electroretinography (ERG) responses in mice at 3 months of age, with nearly complete retina degeneration at 1 year. Removal of CETN2 and CETN3 activity from the lumen of the connecting cilium (CC) destabilized the photoreceptor axoneme and reduced the CC length as early as postnatal day 22 (P22). In Cetn2 (-/-);Cetn3 (GT/GT) double-knockout mice, spermatogenesis-associated 7 (SPATA7), a key organizer of the photoreceptor-specific distal CC, was depleted gradually, and CETN1 was condensed to the mid-segment of the CC. Ultrastructural analysis revealed that in this double knockout, the axoneme of the CC expanded radially at the distal end, with vertically misaligned outer segment discs and membrane whorls. These observations suggest that CETN2 and CETN3 cooperate in stabilizing the CC/axoneme structure. |
