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Allele : Rad1<tm1a(KOMP)Mbp> RAD1 checkpoint DNA exonuclease; targeted mutation 1a, Mouse Biology Program, UC Davis

Primary Identifier  MGI:4843782 Allele Type  Targeted
Attribute String  Conditional ready, Null/knockout, Reporter Gene  Rad1
Transmission  Cell Line Strain of Origin  C57BL/6N-A<tm1Brd>
Is Recombinase  false Is Wild Type  false
Project Collection  KOMP-CSD
molecularNote  The L1L2_Pgk_P cassette was inserted at position 10487612 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 10488467. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
  • mutations:
  • Insertion
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Trail: Allele

Genome

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Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele