Primary Identifier | MGI:3722546 | Allele Type | Targeted |
Attribute String | Not Specified | Gene | Cd28 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to insert alanine substitutions for prolines 187 and 190 in exon 4 (P187A, P190A), thereby disrupting the motif responsible for interaction with SH3 domain-containing proteins. A floxed neo was included with the vector and subsequently removed via cre mediated recombination. |