| First Author | Møller CL | Year | 2011 |
| Journal | Mol Cell Endocrinol | Volume | 341 |
| Issue | 1-2 | Pages | 9-17 |
| PubMed ID | 21616121 | Mgi Jnum | J:183740 |
| Mgi Id | MGI:5319157 | Doi | 10.1016/j.mce.2011.03.010 |
| Citation | Moller CL, et al. (2011) Characterization of murine melanocortin receptors mediating adipocyte lipolysis and examination of signalling pathways involved. Mol Cell Endocrinol 341(1-2):9-17 |
| abstractText | The melanocortin receptors (MCRs) belong to the G-protein coupled receptors (family A). So far, 5 different subtypes have been described (MC1R-MC5R) and of these MC2R and MC5R have been proposed to act directly in adipocytes and regulate lipolysis in rodents. Using ACTH and alpha-melanocyte stimulating hormone (alpha-MSH) generated from proopiomelanocortin (POMC), as well as synthetic MSH analogues to stimulate lipolysis in murine 3T3-L1 adipocytes it is shown that MC2R and MC5R are lipolytic mediators in differentiated 3T3-L1 adipocytes. Involvement of cAMP, phosphorylated extracellular signal-regulated kinase (ERK) 1/2, protein kinase B (PKB), adenosine 5' monophosphate activated protein kinase (AMPK) and Jun-amino-terminal kinase (JNK) in MCR mediated lipolysis were studied. Interestingly, results obtained in 3T3-L1 cells suggest that lipolysis stimulated by alpha-MSH, NDP-alpha-MSH, MT-II, SHU9119 and PG-901 is mediated through MC5R in a cAMP independent manner. Finally, we identify essential differences in MCR mediated lipolysis when using 3T3-L1 cells compared to primary adipocytes. |
