| First Author | Brennan KJ | Year | 1993 |
| Journal | J Biol Chem | Volume | 268 |
| Issue | 1 | Pages | 719-25 |
| PubMed ID | 7678010 | Mgi Jnum | J:131782 |
| Mgi Id | MGI:3774466 | Doi | 10.1016/s0021-9258(18)54211-3 |
| Citation | Brennan KJ, et al. (1993) Quantitative analysis of the human alpha-skeletal actin gene in transgenic mice. J Biol Chem 268(1):719-25 |
| abstractText | Three aspects of the regulation of the human alpha-skeletal actin gene are examined in this study by quantitative analysis of transgenic tissues: level of expression, tissue specificity, and developmental regulation. Previous in vitro and in vivo studies analyzing the 5' end of the gene have indicated that regulation of tissue-specific expression is promoter based. Transgenic mice were produced carrying either a 9.5-kilobase pair (kb) human alpha-skeletal actin gene fragment or a deletion construct with 2.2-kb of 5' sequences of human alpha-skeletal actin linked to the chloramphenicol acetyltransferase reporter gene. We found that the 9.5-kb transgene was capable of expression in adult skeletal muscle at a level equivalent to that of the endogenous gene in a non-transgenic mouse. The deletion construct was also capable of high-level expression. Both transgenes were expressed in a striated muscle-specific manner and were correctly regulated during development. We conclude that these three parameters of regulation of the human alpha-skeletal actin gene are mediated by sequences within the region -2000 to +239 of the promoter. |
