| First Author | Hori Y | Year | 2008 |
| Journal | Cancer Sci | Volume | 99 |
| Issue | 9 | Pages | 1774-7 |
| PubMed ID | 18616525 | Mgi Jnum | J:142480 |
| Mgi Id | MGI:3821561 | Doi | 10.1111/j.1349-7006.2008.00889.x |
| Citation | Hori Y, et al. (2008) Periodic analysis of urethane-induced pulmonary tumors in living A/J mice by respiration-gated X-ray microcomputed tomography. Cancer Sci 99(9):1774-7 |
| abstractText | X-ray microcomputed tomography (micro-CT) with a respiratory gating system is a useful non-invasive approach to evaluate lung tumor development in living animal models. Here micro-CT was applied for the detection of lung lesions induced by a single intraperitoneal injection (250 mg/kg) of urethane in male A/J mice, at 2-week intervals from 10 to 30 weeks after carcinogen exposure. In micro-CT cross sections, lung tumor images were easily distinguished from surrounding non-tumorous tissues, the smallest detected tumor being approximately 0.5 mm in diameter. All of the urethane-treated mice (n = 15) developed lung tumors and the number of tumors developed in each mouse was 8.6 +/- 3.9. Six tumors, determined histopathologically to be adenocarcinomas, were detected, growing at different rates during the experimental period. The most aggressive carcinoma, increasing in diameter from 0.9 to 3.5 mm within 8 weeks, was a solid-type nodule with a clear tumor margin on the micro-CT imaging. Other tumors, histopathologically adenomas, grew slowly or moderately. The results provide evidence that micro-CT is a useful non-invasive imaging approach for evaluating the characteristics and growth of lung tumors in mice. |
