| First Author | Porter-Stransky KA | Year | 2019 |
| Journal | Biol Psychiatry | Volume | 85 |
| Issue | 3 | Pages | 237-247 |
| PubMed ID | 30269865 | Mgi Jnum | J:287195 |
| Mgi Id | MGI:6391557 | Doi | 10.1016/j.biopsych.2018.07.027 |
| Citation | Porter-Stransky KA, et al. (2019) Noradrenergic Transmission at Alpha1-Adrenergic Receptors in the Ventral Periaqueductal Gray Modulates Arousal. Biol Psychiatry 85(3):237-247 |
| abstractText | BACKGROUND: Dysregulation of arousal is symptomatic of numerous psychiatric disorders. Previous research has shown that the activity of dopamine (DA) neurons in the ventral periaqueductal gray (vPAG) tracks with arousal state, and lesions of vPAG(DA) cells increase sleep. However, the circuitry controlling these wake-promoting DA neurons is unknown. METHODS: This study combined designer receptors exclusively activated by designer drugs (DREADDs), behavioral pharmacology, electrophysiology, and immunoelectron microscopy in male and female mice to elucidate mechanisms in the vPAG that promote arousal. RESULTS: Activation of locus coeruleus projections to the vPAG or vPAG(DA) neurons induced by DREADDs promoted arousal. Similarly, agonist stimulation of vPAG alpha1-adrenergic receptors (alpha1ARs) increased latency to fall asleep, whereas alpha1AR blockade had the opposite effect. alpha1AR stimulation drove vPAG(DA) activity in a glutamate-dependent, action potential-independent manner. Compared with other dopaminergic brain regions, alpha1ARs were enriched on astrocytes in the vPAG, and mimicking alpha1AR transmission specifically in vPAG astrocytes via Gq-DREADDS was sufficient to increase arousal. In general, the wake-promoting effects observed were not accompanied by hyperactivity. CONCLUSIONS: These experiments revealed that vPAG alpha1ARs increase arousal, promote glutamatergic input onto vPAG(DA) neurons, and are abundantly expressed on astrocytes. Activation of locus coeruleus inputs, vPAG astrocytes, or vPAG(DA) neurons increase sleep latency but do not produce hyperactivity. Together, these results support an arousal circuit whereby noradrenergic transmission at astrocytic alpha1ARs activates wake-promoting vPAG(DA) neurons via glutamate transmission. |
