| First Author | Chen Z | Year | 1994 |
| Journal | Genes Dev | Volume | 8 |
| Issue | 19 | Pages | 2293-301 |
| PubMed ID | 7958896 | Mgi Jnum | J:20882 |
| Mgi Id | MGI:68949 | Doi | 10.1101/gad.8.19.2293 |
| Citation | Chen Z, et al. (1994) Transcriptional enhancer factor 1 disruption by a retroviral gene trap leads to heart defects and embryonic lethality in mice. Genes Dev 8(19):2293-301 |
| abstractText | We have used a retroviral gene trap in embryonic stem (ES) cells to derive a recessive embryonic lethal mouse strain, ROSA beta-geo5. Mutant embryos display an enlarged pericardial cavity, bradycardia, a dilated fourth ventricle in the brain, and die between embryonic days 11 and 12. Whereas heart development in the mutant embryos is extensive, the ventricular wall is abnormally thin with a reduced number of trabeculae. Cloning of the trapped gene indicates that proviral insertion creates a null mutation in the transcriptional enhancer factor 1 (TEF-1) gene. Although transcription of a number of muscle-specific genes believed to be TEF-1 targets appears normal, the defect in cardiogenesis is likely attributable to diminished transcription of one or several cardiac-specific genes. |
