| First Author | Williams DE | Year | 1990 |
| Journal | Cell | Volume | 63 |
| Issue | 1 | Pages | 167-74 |
| PubMed ID | 1698553 | Mgi Jnum | J:10747 |
| Mgi Id | MGI:59193 | Doi | 10.1016/0092-8674(90)90297-r |
| Citation | Williams DE, et al. (1990) Identification of a ligand for the c-kit proto-oncogene. Cell 63(1):167-74 |
| abstractText | We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proliferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit protooncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein. |
