| First Author | Ali PS | Year | 2012 |
| Journal | FEBS Lett | Volume | 586 |
| Issue | 22 | Pages | 3986-90 |
| PubMed ID | 23063642 | Mgi Jnum | J:190720 |
| Mgi Id | MGI:5449504 | Doi | 10.1016/j.febslet.2012.09.034 |
| Citation | Ali PS, et al. (2012) Recognition of the let-7g miRNA precursor by human Lin28B. FEBS Lett 586(22):3986-90 |
| abstractText | Mammalian homologs of lin28: Lin28 and Lin28B block the post-transcriptional processing of the let-7 family of miRNAs. We report that in vitro the terminal stem-loop region of the let-7g miRNA precursor (pre-let-7g) required to bind Lin28B is restricted to 24 nucleotides (nt) including the 3' GGAG motif. Additionally, full length Lin28B is required for efficient binding to pre-let-7g and the stoichiometry of the complex is 1:1. Molecular dynamics (MD) simulations reveal the interactions of the pre-let-7g stem-loop and the GGAG motif in the stem region to the cold shock domain (CSD) and to the zinc knuckle domain (ZKD) of Lin28B, respectively. |
