| First Author | Saegusa C | Year | 2006 |
| Journal | Genes Cells | Volume | 11 |
| Issue | 6 | Pages | 623-31 |
| PubMed ID | 16716193 | Mgi Jnum | J:116278 |
| Mgi Id | MGI:3693417 | Doi | 10.1111/j.1365-2443.2006.00964.x |
| Citation | Saegusa C, et al. (2006) Decreased basal mucus secretion by Slp2-a-deficient gastric surface mucous cells. Genes Cells 11(6):623-31 |
| abstractText | Synaptotagmin-like protein (Slp) 2-a is a putative Rab27A/B-effector protein and is implicated in intracellular membrane transport. However, the precise tissue distribution of Slp2-a protein and its functions remain largely unknown. In this study we used a specific anti-Slp2-a antibody to investigate the tissue distribution of Slp2-a in mice and found that Slp2-a is most abundantly expressed in mouse stomach. Co-immunoprecipitation experiments indicated that Slp2-a interacts with Rab27A/B in vivo. We also discovered that Slp2-a and Rab27A/B are predominantly localized at the apical region of gastric-surface mucous cells, where mucus granules are accumulated. Analysis of Slp2-a mutant mice generated by homologous recombination showed a reduced number of mucus granules, a deficiency of granule docking with the apical plasma membrane in the gastric-surface mucous cells and reduction of mucus secretion by Slp2-a-deficient gastric primary cells. Based on these results, we propose that Slp2-a is part of the mucin secretory machinery in surface mucous cells of mouse stomach. |
