| First Author | Kishigami S | Year | 2006 |
| Journal | Genesis | Volume | 44 |
| Issue | 2 | Pages | 57-65 |
| PubMed ID | 16419090 | Mgi Jnum | J:106694 |
| Mgi Id | MGI:3619271 | Doi | 10.1002/gene.20186 |
| Citation | Kishigami S, et al. (2006) Optimized beta-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos. Genesis 44(2):57-65 |
| abstractText | beta-Galactosidase (beta-gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6-chloro-3-indoxyl-beta-D-galactopyranoside (S-gal) is more sensitive for beta-gal activity than 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined beta-gal staining with S-gal and in situ hybridization using DIG-labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S-gal. |
