Primary Identifier | MGI:2181803 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Mest |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Sequence encompassing all of exons 3 through 8 and a portion of exon 9 was replaced with a cassette containing an IRES-lacZ-neo fusion gene. RT-PCR analysis of total embryonic RNA showed an absence of normal transcript in animals possessing a targeted paternal allele and a silenced wild-type maternal allele. Paternal transmission of this allele yielded a lacZ expression pattern similar to that of the endogenous gene. |