| First Author | Bowles J | Year | 2000 |
| Journal | Genesis | Volume | 27 |
| Issue | 3 | Pages | 124-35 |
| PubMed ID | 10951505 | Mgi Jnum | J:64333 |
| Mgi Id | MGI:1889110 | Citation | Bowles J, et al. (2000) A subtractive gene expression screen suggests a role for vanin-1 in testis development in mice. Genesis 27(3):124-35 |
| abstractText | The molecular pathways leading from indifferent mammalian gonad to either testis or ovary are not well understood. A number of genes, including the Y-linked sex determining gene SRY, have been shown to play roles in sex determination or differentiation, but there are clearly many missing elements to be found. We used suppression-subtractive hybridization to construct normalized cDNA libraries enriched for male-specific or female-specific transcripts in mouse fetal gonads. We describe the strategy used to efficiently screen these libraries for candidate sex-determination and gonadogenesis genes. One gene arising from these screens is vanin-1, which encodes a protein implicated in the induction of cell migration into the thymus. We find that vanin-1 is expressed male-specifically in Sertoli cells of the developing testis and may be involved in inducing cell migration from the adjacent mesonephros, a process known to be critical for testis development. This screening approach is likely to be applicable to the isolation and study of genes involved in a variety of developmental systems. |
