| First Author | Borchert A | Year | 1999 |
| Journal | FEBS Lett | Volume | 446 |
| Issue | 2-3 | Pages | 223-7 |
| PubMed ID | 10100845 | Mgi Jnum | J:53501 |
| Mgi Id | MGI:1332867 | Doi | 10.1016/s0014-5793(99)00221-5 |
| Citation | Borchert A, et al. (1999) Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene. FEBS Lett 446(2-3):223-7 |
| abstractText | 15-Lipoxygenases and phospholipid hydroperoxide glutathione peroxidases (PH-GPx) are counterparts in the metabolism of hydroperoxy lipids and a balanced regulation of both enzymes appears to be important for the cellular peroxide tone regulating the expression of redox sensitive genes. In contrast to lipoxygenases the molecular biology of PH-GPx is less well investigated. In this study we cloned the PH-GPx cDNA from a mouse fibroblast cDNA library and the PH-GPx gene from a mouse genomic library. The gene spans approximately 4 kb which includes 1 kb of 5'-flanking region and consists of seven exons and six introns. The immediate promoter region does not contain a TATA box but there are binding sites for several transcription factors which also occur in the porcine gene. Our investigations provide useful tools for future targeted gene disruption studies. |
