| First Author | Mittrücker HW | Year | 2001 |
| Journal | J Immunol | Volume | 167 |
| Issue | 10 | Pages | 5620-7 |
| PubMed ID | 11698433 | Mgi Jnum | J:72680 |
| Mgi Id | MGI:2153393 | Doi | 10.4049/jimmunol.167.10.5620 |
| Citation | Mittrucker HW, et al. (2001) Role of CD28 for the Generation and Expansion of Antigen-Specific CD8(+) T Lymphocytes During Infection with Listeria monocytogenes. J Immunol 167(10):5620-7 |
| abstractText | Infection of mice with the intracellular bacterium Listeria monocytogenes results in a strong CD8(+) T cell response that is critical for efficient control of infection. We used CD28-deficient mice to characterize the function of CD28 during Listeria infection, with a main emphasis on Listeria-specific CD8(+) T cells. Frequencies and effector functions of these T cells were determined using MHC class I tetramers, single cell IFN-gamma production and Listeria-specific cytotoxicity. During primary Listeria infection of CD28(-/-) mice we observed significantly reduced numbers of Listeria-specific CD8(+) T cells and only marginal levels of specific IFN-gamma production and cytotoxicity. Although frequencies were also reduced in CD28(-/-) mice during secondary response, we detected a considerable population of Listeria-specific CD8(+) T cells in these mice. In parallel, IFN-gamma production and cytotoxicity were observed, revealing that Listeria-specific CD8(+) T cells in CD28(-/-) mice expressed normal effector functions. Consistent with their impaired CD8(+) T cell activation, CD28(-/-) mice suffered from exacerbated listeriosis both after primary and secondary infection. These results demonstrate participation of CD28 signaling in the generation and expansion of Ag-specific CD8(+) T cells in listeriosis. However, Ag-specific CD8(+) T cells generated in the absence of CD28 differentiated into normal effector and memory T cells. |
