| First Author | Miwa A | Year | 2006 |
| Journal | Biochim Biophys Acta | Volume | 1760 |
| Issue | 11 | Pages | 1675-81 |
| PubMed ID | 17045744 | Mgi Jnum | J:116992 |
| Mgi Id | MGI:3695347 | Doi | 10.1016/j.bbagen.2006.08.027 |
| Citation | Miwa A, et al. (2006) Expression and polysome association of YB-1 in various tissues at different stages in the lifespan of mice. Biochim Biophys Acta 1760(11):1675-81 |
| abstractText | Tissue-specific translational regulation is important for gene expression. YB-1 binds to mRNAs to form mRNPs and affects translation. In this study we investigated expression and polysome association of YB-1 in various tissues at different stages in the lifespan of mice. YB-1 levels decreased markedly with growth in brain, heart and muscle, but increased in the spleen. In lung, kidney and testis, the levels of YB-1 diminished with aging. In liver, no significant change in the level of YB-1 was observed throughout life. We further showed that the distribution pattern of YB-1 on a sucrose gradient differed according to tissue. Moreover, the distribution pattern of YB-1 changed drastically with growth in the liver. In 5-day-old liver, YB-1 was distributed almost exclusively in nonpolysomal fractions, whereas in 4-week-old liver, it was associated with heavy-sedimenting polysomes, as was the case in 5-day-old brain. Immunohistochemical analysis revealed that YB-1 is mainly a cytoplasmic protein in these tissues. Our results indicate that the expression and polysome association of YB-1 are regulated with growth or aging in a tissue-specific manner, presumably to control gene expression at the translational level in each tissue. |
