Primary Identifier | MGI:7346402 | Allele Type | Endonuclease-mediated |
Attribute String | Modified regulatory region, Null/knockout | Gene | Fmn1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An enhancer cluster containing Grem1 limb regulatory regions Rr285 (CRM2), Rr284 (CRM3) and Rr26 (CRM4), located in Fmn1 introns 15 and 13 [in reference to ENSMUST00000081349], was targeted with sgRNAs (targeting CACCGTGGCTTACCAGACTAGCGGT and CACCGAATGGTCTGATCGCCA) using CRISPR/Cas9 technology, resulting in a 71.2 kb deletion (chr2:113467121-113538319 GRCm39) from intron 13 to 16 (so including exons 14-16). |