Primary Identifier | MGI:6272744 | Allele Type | Endonuclease-mediated |
Attribute String | Modified isoform(s), Null/knockout | Gene | Syne1 |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Exon -64 (counted from the last exon backwards) was targeted for the insertion of a 16 bp sequence with an sgRNA and a template oligonucleotide using CRSIPR/Cas9 technology. The inserted sequence contains stop codons in all three reading frames and a diagnostic EcoRI restriction site and was inserted after glutamine codon 5328, 131 bp into the exon. The result is an allele where the Nes1g (nesprin 1 giant) splice variants have a premature stop codon. Immunoblots showed a complete absence of giant isoforms in brain lysates. |