| First Author | Testa G | Year | 2004 |
| Journal | Genesis | Volume | 38 |
| Issue | 3 | Pages | 151-8 |
| PubMed ID | 15048813 | Mgi Jnum | J:138283 |
| Mgi Id | MGI:3804746 | Doi | 10.1002/gene.20012 |
| Citation | Testa G, et al. (2004) A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles. Genesis 38(3):151-8 |
| abstractText | Alteration of the mouse genome through homologous recombination in embryonic stem (ES) cells is the most accurate and versatile way to dissect gene function in a vertebrate model. Most often, a selectable marker is used to create a knockout allele by replacing an essential part of the gene. However, knockout strategies are limited because the mutation is present constitutively. Conditional approaches based on the Cre-loxP site-specific recombination (SSR) system address this limitation; however, it requires that all parts of the targeted gene remain in ES cells. Here we report success with a 'knockout-first' strategy that ablates gene function by insertion of RNA processing signals without deletion of any of the target gene. Incorporation of site-specific recombination target sites creates a multipurpose allele for both knockout and conditional applications. |
