| First Author | Miyasaka T | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 10 | Pages | e78611 |
| PubMed ID | 24205278 | Mgi Jnum | J:291653 |
| Mgi Id | MGI:6236569 | Doi | 10.1371/journal.pone.0078611 |
| Citation | Miyasaka T, et al. (2013) Dectin-2-dependent NKT cell activation and serotype-specific antibody production in mice immunized with pneumococcal polysaccharide vaccine. PLoS One 8(10):e78611 |
| abstractText | Although thymus-independent type 2 antigens generally do not undergo Ig class switching from IgM to IgG, pneumococcal polysaccharide vaccine (PPV) induces the production of serotype-specific IgG. How this happens remains unclear, however. In the present study, PPV immunization induced production of IgG as well as IgM specific for a serotype 3-pneumococcal polysaccharide in the sera of wild-type (WT) mice, but this phenomenon was significantly reduced in Dectin-2 knockout (KO) mice. Immunization with PPV caused IL-12p40 production in WT mice, but this response was significantly reduced in Dectin-2KO mice. Likewise, immunization with PPV activated natural killer T (NKT) cells in WT mice but not in Dectin-2KO mice. Furthermore, administration of alpha-galactosylceramide, recombinant (r)IL-12 or rIFN-gamma improved the reduced IgG levels in Dectin-2KO mice, and treatment with neutralizing anti-IFN-gamma mAb resulted in the reduction of IgG synthesis in PPV-immunized WT mice. Transfer of spleen cells from PPV-immunized WT mice conferred protection against pneumococcal infection on recipient mice, whereas this effect was cancelled when the transferred spleen cells were harvested from PPV-immunized Dectin-2KO mice. These results suggest that the detection of PPV antigens via Dectin-2 triggers IL-12 production, which induces IFN-gamma synthesis by NKT cells and subsequently the production of serotype-specific IgG. |
