| First Author | Chung SS | Year | 2010 |
| Journal | Mol Cell Biol | Volume | 30 |
| Issue | 9 | Pages | 2135-46 |
| PubMed ID | 20194620 | Mgi Jnum | J:161702 |
| Mgi Id | MGI:4461072 | Doi | 10.1128/MCB.00852-09 |
| Citation | Chung SS, et al. (2010) Control of adipogenesis by the SUMO-specific protease SENP2. Mol Cell Biol 30(9):2135-46 |
| abstractText | Here, we demonstrate that SENP2, a desumoylating enzyme, plays a critical role in the control of adipogenesis. SENP2 expression was markedly increased upon the induction of adipocyte differentiation, and this increase was dependent on protein kinase A activation. Remarkably, knockdown of SENP2 led to a dramatic attenuation of adipogenesis with a marked decrease in PPARgamma and C/EBPalpha mRNA levels. Knockdown of SENP2 also caused a marked reduction in the level of C/EBPbeta protein but not in that of C/EBPbeta mRNA. Interestingly, sumoylation of C/EBPbeta dramatically increased its ubiquitination and destabilization, and this increase could be reversed by SENP2. In addition, overexpression of C/EBPbeta could overcome the inhibitory effect of SENP2 knockdown on adipogenesis. Furthermore, SENP2 was absolutely required for adipogenesis of preadipocytes implanted into mice. These results establish a critical role for SENP2 in the regulation of adipogenesis by desumoylation and stabilization of C/EBPbeta and in turn by promoting the expression of its downstream effectors, such as PPARgamma and C/EBPalpha. |
