| First Author | Negoro T | Year | 2014 |
| Journal | Mol Immunol | Volume | 57 |
| Issue | 2 | Pages | 141-50 |
| PubMed ID | 24084100 | Mgi Jnum | J:206025 |
| Mgi Id | MGI:5547669 | Doi | 10.1016/j.molimm.2013.08.015 |
| Citation | Negoro T, et al. (2014) Potentiated macrophage activation by acid sensing under low adiponectin levels. Mol Immunol 57(2):141-50 |
| abstractText | Adiponectin can protect against inflammation; one of the mechanisms involves direct, inhibition of macrophages (MPhi). We postulated that adiponectin anti-sense transgenic (AsTg) mice raised in our laboratory are prone to inflammation because of systemic low adiponectin levels. The writhing response to acetic acid was utilized as an in vivo inflammatory model, and using Ca(2)(+), response to the acid was exploited in vitro to evaluate the function of resident peritoneal MPhi. The in vivo response to the acid was increased and the Ca(2)(+) response of MPhi was enhanced in AsTg mice, compared with those in wild type (WT) mice. In parallel with these enhanced responses, MPhi from AsTg mice augmented TNF-alpha and IL-6 mRNA expression. We further analyzed the enhancement in activity of MPhi from AsTg mice by acid sensing using specific inhibitors, amiloride for acid-sensing ion channels (ASICs) and KB-R7943 for Na(+)/Ca(2)(+) exchangers (NCXs). Our results indicated that in AsTg mice, the Ca(2)(+) response to the acid was facilitated in MPhi by a low threshold of ASIC1 and NCX1 molecules and the activity of these channel was possibly regulated by adiponectin. |
