| Primary Identifier | MGI:4950066 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gnas |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cassette containing a polyadenylation sequence in the same orientation as Nespas and a self-excising neo cassette was inserted into exon 1. Cre-mediated recombination removed the neo cassette. The absence of Nespas transcript expression was confirmed by RT-qPCR on newborn brain extracts with paternal inheritance of the allele. De-repression of Gnas was confirmed by RT-qPCR. |
