Primary Identifier | MGI:6469881 | Allele Type | Targeted |
Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Ace2 |
Transmission | Germline | Strain of Origin | 129S2/SvPas |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct is designed to replace exon 2 with a human ACE2 cDNA inserted in-frame with the endogenous initiation codon. in addition, a loxP site is placed upstream of the ACE2 cDNA and a FRT-flanked neomycin resistance (neo) cassette and a second loxP site is placed downstream of the ACE2 cDNA. Flp-mediated recombination removed the FRT-flanked neo cassette. |