Primary Identifier | MGI:6196853 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct encoding a loxP-flanked transcriptional STOP cassette upstream of chicken ovalbumin lacking its signal sequence fused to the C-terminal 166 amino acids of Legionella pneumophila flagellin and an IRES-GFP downstream of the fusion gene was targeted to the locus. This creates a conditional-ready allele that will only be expressed after cre-mediated deletion of the STOP cassette. Expression of flagellin activates endogenous NAIP/NLLRC4 inflammasome while the ovalbumin permits the tracking of the adaptive immune response. |