| First Author | Klattenhoff CA | Year | 2013 |
| Journal | Cell | Volume | 152 |
| Issue | 3 | Pages | 570-83 |
| PubMed ID | 23352431 | Mgi Jnum | J:207590 |
| Mgi Id | MGI:5559147 | Doi | 10.1016/j.cell.2013.01.003 |
| Citation | Klattenhoff CA, et al. (2013) Braveheart, a long noncoding RNA required for cardiovascular lineage commitment. Cell 152(3):570-83 |
| abstractText | Long noncoding RNAs (lncRNAs) are often expressed in a development-specific manner, yet little is known about their roles in lineage commitment. Here, we identified Braveheart (Bvht), a heart-associated lncRNA in mouse. Using multiple embryonic stem cell (ESC) differentiation strategies, we show that Bvht is required for progression of nascent mesoderm toward a cardiac fate. We find that Bvht is necessary for activation of a core cardiovascular gene network and functions upstream of mesoderm posterior 1 (MesP1), a master regulator of a common multipotent cardiovascular progenitor. We also show that Bvht interacts with SUZ12, a component of polycomb-repressive complex 2 (PRC2), during cardiomyocyte differentiation, suggesting that Bvht mediates epigenetic regulation of cardiac commitment. Finally, we demonstrate a role for Bvht in maintaining cardiac fate in neonatal cardiomyocytes. Together, our work provides evidence for a long noncoding RNA with critical roles in the establishment of the cardiovascular lineage during mammalian development. |
