Primary Identifier | MGI:3514149 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Dmbt1 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The first exon and 300 bp of the first intron were replaced by a cassette composed of a GFP cassette inserted downstream of the TATA box in such a way that the GFP start codon replaced that of the endogenous gene as well as a PGK neomycin gene in reverse orientation. Lack of expression was confirmed by QRT-PCR and Western blot analysis. No GFP protein expression or mRNA was detected. |