Primary Identifier | MGI:2671049 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Igsf1 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 1 was replaced with a PGK-HPRT cassette. This deletes the transcription start site used by three isoforms of this gene. Northern blot analysis indicated that mRNA for these isoforms was not present. mRNA for a fourth isoform which uses a different transcription start site was detectable however. These results were confirmed by RT-PCR. |