| First Author | Kato Y | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 275 |
| Issue | 1 | Pages | 11-5 |
| PubMed ID | 10944432 | Mgi Jnum | J:64044 |
| Mgi Id | MGI:1888630 | Doi | 10.1006/bbrc.2000.3265 |
| Citation | Kato Y, et al. (2000) Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody. Biochem Biophys Res Commun 275(1):11-5 |
| abstractText | Dityrosine is one of the specific biomarkers for proteinoxidation. We prepared an antibody specific for proteindityrosine using a dimer of 3-p-(hydroxyphenyl)propionic acid (di-HP) as a hapten. Three clones (A8, G6, and 1C3) were obtained, and the antibody from the A8 clone reacted with the di-HP-conjugated protein but not with a dityrosine conjugate. The others (G6 and 1C3 clones) recognized both the di-HP and dityrosine conjugates. The antibodies reacted with peptidyl dityrosine, derived from Thr-Tyr-Ser, rather than the free dityrosine. The reactivity of the latter two antibodies with lens proteins oxidized by incubation with H(2)O(2)/Cu was in good accordance with the formation of the dityrosine-like fluorescence. Using the obtained monoclonal antibody, the immunopositive staining of atherosclerotic lesions in Apo E-deficient mice was confirmed by an immunohistochemical technique. Copyright 2000 Academic Press. |
