| First Author | Honjo K | Year | 2004 |
| Journal | Transplantation | Volume | 77 |
| Issue | 3 | Pages | 452-5 |
| PubMed ID | 14966425 | Mgi Jnum | J:155849 |
| Mgi Id | MGI:4415808 | Doi | 10.1097/01.TP.0000112937.12491.42 |
| Citation | Honjo K, et al. (2004) CD4+ T-cell receptor transgenic T cells alone can reject vascularized heart transplants through the indirect pathway of alloantigen recognition. Transplantation 77(3):452-5 |
| abstractText | The vast array of epitopes presented by allografts and the diversity of T cells responding to them complicates mechanistic studies of rejection. To minimize these problems, we developed a transgenic (Tg) model system limited to a single T-cell receptor (TCR)/peptide/major histocompatibility complex molecule. Two alloantigen-specific CD4 T-cell clones were used to isolate cDNA encoding the TCRalpha and TCRbeta chains that recognize the Kd54-68/I-Ab epitope. Two different TCR Tg lines were produced in C57BL/6 (B6) mice and crossed onto the B6.Rag1-/- background. B6.Rag1-/- recipients of T cells from TCR Tg Rag1-/-mice promptly rejected B10.D2, but not irrelevant B10.BR, cardiac grafts. Thus, a single allogeneic epitope presented by self-major histocompatibility complex class II is sufficient to activate TCR Tg T cells and serve as a target for rejection. |
