First Author | Hallenberg C | Year | 1994 |
Journal | Gene | Volume | 142 |
Issue | 2 | Pages | 291-5 |
PubMed ID | 8194767 | Mgi Jnum | J:18337 |
Mgi Id | MGI:66337 | Doi | 10.1016/0378-1119(94)90277-1 |
Citation | Hallenberg C, et al. (1994) Characterization of 5S rRNA genes from mouse. Gene 142(2):291-5 |
abstractText | In order to characterize the transcriptional regulation of the 5S rRNA genes we have isolated a bona fide gene and a pseudogene from mouse cells. These 5S rRNA genes contain a 12-bp sequence designated as the D-box, located in position -33 to -22 bp, and two Sp1-binding sites in the 5'-flanking region. The D-box is conserved in human and hamster 5S rRNA genes although in slightly different upstream positions. The bona fide mouse 5S rRNA gene was transcribed in a HeLa S-100 extract. The transcriptional activity of this gene was only 50% of that of the human gene, indicating the involvement of species-specific transcription factors and/or polymerases. The pseudogene which contains the D-box, but with position +25 to +35 bp deleted, showed no transcriptional activity. Deletion of the D-box in the 5'-flanking sequence abolished transcriptional activity, indicating that this conserved sequence is of importance for gene expression. |