| Primary Identifier | MGI:4868581 | Allele Type | Targeted |
| Gene | Lmna | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exons 10, 11, and 12 (and the introns between) were replaced with the corresponding cDNA sequence in which the last 150 bp of exon 11 were removed as well as 3 nucleotides of exon 12 that code for the isoleucine in the C-terminal CSIM sequence. A floxed neo cassette was inserted downstream of exon 12. This allele is predicted to produce a non-farnesylated protein because it lacks the farnesylatible isoleucine in the CaaX motif. The absence of A and C isoforms and production of the progerin isoform was confirmed by western blot analysis on liver extracts. |
