| First Author | Wu Y | Year | 2023 |
| Journal | Sci Adv | Volume | 9 |
| Issue | 16 | Pages | eade7622 |
| PubMed ID | 37075105 | Mgi Jnum | J:337494 |
| Mgi Id | MGI:7465836 | Doi | 10.1126/sciadv.ade7622 |
| Citation | Wu Y, et al. (2023) Simultaneous Fe(2+)/Fe(3+) imaging shows Fe(3+) over Fe(2+) enrichment in Alzheimer's disease mouse brain. Sci Adv 9(16):eade7622 |
| abstractText | Visualizing redox-active metal ions, such as Fe(2+) and Fe(3+) ions, are essential for understanding their roles in biological processes and human diseases. Despite the development of imaging probes and techniques, imaging both Fe(2+) and Fe(3+) simultaneously in living cells with high selectivity and sensitivity has not been reported. Here, we selected and developed DNAzyme-based fluorescent turn-on sensors that are selective for either Fe(2+) or Fe(3+), revealing a decreased Fe(3+)/Fe(2+) ratio during ferroptosis and an increased Fe(3+)/Fe(2+) ratio in Alzheimer's disease mouse brain. The elevated Fe(3+)/Fe(2+) ratio was mainly observed in amyloid plaque regions, suggesting a correlation between amyloid plaques and the accumulation of Fe(3+) and/or conversion of Fe(2+) to Fe(3+). Our sensors can provide deep insights into the biological roles of labile iron redox cycling. |
