| First Author | Pan YX | Year | 1996 |
| Journal | Biochem J | Volume | 315 ( Pt 1) |
| Pages | 11-3 | PubMed ID | 8670093 |
| Mgi Jnum | J:32541 | Mgi Id | MGI:80035 |
| Doi | 10.1042/bj3150011 | Citation | Pan YX, et al. (1996) Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor. Biochem J 315(Pt 1):11-3 |
| abstractText | By using a reverse transcription-PCR approach we have cloned a peptide precursor from mouse brain which contains the sequence of orphanin FQ/nociceptin. The mouse sequence of orphanin FQ/nociceptin is identical at the amino acid level with that isolated from rat and porcine brain. Northern analysis of the mRNA encoding the precursor reveals a single band of approx. 1 kb, with the highest levels in the brain and much lower levels in kidney and spleen. Southern analysis is consistent with a single gene. The precursor peptide from mouse contains two putative peptides. Upstream from the orphanin FQ/nociceptin is a 41-amino-acid peptide which is almost identical, except for a six-amino-acid insertion, with the corresponding 35-amino-acid peptide predicted from the rat sequence. Interestingly, the mouse contains a triple AEPGAD repeat within this peptide that is not seen in the rat sequence. Downstream from the orphanin FQ/nociceptin sequence is another 17-amino-acid peptide which is identical with that found in the rat. |
