| Experiment Id | GSE155794 | Name | Identification and Characterization of Cellular Heterogeneity within the Developing Renal Interstitium |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2024-03-29 |
| description | Kidney formation requires the coordinated growth of multiple cell types including the collecting ducts, nephrons, vasculature and interstitium. We show that during development, the interstitium is a highly heterogeneous, patterned population of cells that occupies distinct positions correlated to the adjacent parenchyma. Our analysis indicates that the heterogeneity is not a mere reflection of different stages in a linear developmental trajectory but instead represents several novel differentiated cell states. Further, we find that beta-catenin has a cell autonomous role in the development of a medullary subset of the interstitium and that this non-autonomously affects the development of the adjacent epithelia. We performed single cell RNA sequencing on Foxd1-positive cells purified by fluorescence activated cell sorting (FACS) from Foxd1Cre;Rosa-Tomato E18.5 mouse kidneys. |
