| First Author | Keegan BP | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 267 |
| Issue | 3 | Pages | 881-6 |
| PubMed ID | 10673385 | Mgi Jnum | J:60172 |
| Mgi Id | MGI:1352937 | Doi | 10.1006/bbrc.1999.2034 |
| Citation | Keegan BP, et al. (2000) The internalization and endosomal trafficking of the EGF receptor in response to EGF is delayed in the waved-2 mouse liver. Biochem Biophys Res Commun 267(3):881-6 |
| abstractText | The EGF receptor in waved-2 mice contains a point mutation that renders it kinase-deficient. We investigated how the waved-2 mutation affects the internalization and endosomal trafficking of the receptor in vivo in response to EGF. When the waved-2 mice were injected with EGF, there was approximately 50% less tyrosine phosphorylation detected in whole-liver homogenate compared to wild-type background mice. Although EGF increased the EGF receptor levels in the early and late liver endosomal fractions of waved-2 mice, its trafficking was delayed compared to wild-type mice. Ubiquitination of the EGF receptor may affect its endosomal sorting. We found that a similar amount of EGF receptor was immunoprecipitated from the endosomal fractions of EGF-treated waved-2 and wild-type with anti-ubiquitin antibody. These results demonstrate that the waved-2 EGF receptor can become ubiquitinated and can be trafficked to the late endosomes, although it appears that its kinase deficiency delays this process. Copyright 2000 Academic Press. |
