| Primary Identifier | MGI:2156895 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Dnmt1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| description | Mouse embryo fibroblasts derived from chimeric mice made with Dnmt1tm2Jae/tm4Jae ES cells in which imprinting had been eliminated (J:104860) display a significantly faster growth rate with nearly half (47%) of cells in the G2 or M phase of the cell cycle. These MEF cells become spontaneously immortalized. The chimeric mice experience widespread tumorigenesis. |
| molecularNote | Homologous recombination inserted a floxed CMV-hygromycin-thymidine kinase expression cassette downstream of exon 5 and placed a loxP site in intron 3. Cre-mediated recombination in ES cells excised the expression cassette and left loxP sites flanking exons 4 and 5, which encode the localization motifs and the entire catalytic domain of the protein. |
