| First Author | Rutkowsky T | Year | 2014 |
| Journal | Methods Mol Biol | Volume | 1130 |
| Pages | 193-201 | PubMed ID | 24482174 |
| Mgi Jnum | J:210091 | Mgi Id | MGI:5569552 |
| Doi | 10.1007/978-1-62703-989-5_14 | Citation | Rutkowsky T, et al. (2014) Whole-mount in situ hybridization on murine skeletogenic tissues. Methods Mol Biol 1130:193-201 |
| abstractText | Whole-mount in situ hybridization (WISH) is a highly sensitive method for spatial and temporal visualization of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying both embryonic development and disease. It is based on sequence complementation between a Digoxigenin (DIG)-labeled antisense RNA probe and the sense RNA transcript of interest in the tissue. The labeled probe is then visualized by immunohistochemical methods using an antibody conjugated to alkaline phosphatase that catalyzes a color reaction. |
