| First Author | Deák F | Year | 2004 |
| Journal | Nat Cell Biol | Volume | 6 |
| Issue | 11 | Pages | 1102-8 |
| PubMed ID | 15475946 | Mgi Jnum | J:96873 |
| Mgi Id | MGI:3573806 | Doi | 10.1038/ncb1185 |
| Citation | Deak F, et al. (2004) Synaptobrevin is essential for fast synaptic-vesicle endocytosis. Nat Cell Biol 6(11):1102-8 |
| abstractText | Synaptobrevin-2 (VAMP-2), the major SNARE protein of synaptic vesicles, is required for fast calcium-triggered synaptic-vesicle exocytosis. Here we show that synaptobrevin-2 is also essential for fast synaptic-vesicle endocytosis. We demonstrate that after depletion of the readily releasable vesicle pool, replenishment of the pool is delayed by knockout of synaptobrevin. This delay was not from a loss of vesicles, because the total number of pre-synaptic vesicles, docked vesicles and actively recycling vesicles was unaffected. However, altered shape and size of the vesicles in synaptobrevin-deficient synapses suggests a defect in endocytosis. Consistent with such a defect, the stimulus-dependent endocytosis of horseradish peroxidase and fluorescent FM1-43 were delayed, indicating that fast vesicle endocytosis may normally be nucleated by a SNARE-dependent coat. Thus, synaptobrevin is essential for two fast synapse-specific membrane trafficking reactions: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid reuse of synaptic vesicles. |
