| First Author | Hodges MD | Year | 2002 |
| Journal | Genomics | Volume | 80 |
| Issue | 5 | Pages | 531-42 |
| PubMed ID | 12408971 | Mgi Jnum | J:80100 |
| Mgi Id | MGI:2429801 | Doi | 10.1006/geno.2002.6854 |
| Citation | Hodges MD, et al. (2002) Characterization of the genomic and transcriptional structure of the CRX gene: substantial differences between human and mouse. Genomics 80(5):531-42 |
| abstractText | We have previously shown that there is a temporal difference in human CRX: gene expression compared with that of mouse Crx. We have now characterized these genes at the genomic and transcriptional levels and here we expand on this earlier report. Human CRX: spans 25 kb and has six exons, and mouse CRX: spans 15 kb and has four exons. We isolated seven human and two mouse mRNAs generated by alternative splicing of a variable 5' untranslated region. The human and mouse genes share an evolutionarily conserved promoter, which contains OTX/CRX type and SP1/AP2 binding sites and drives expression of two conserved transcripts in both species. Additionally, the human gene has a second human-specific promoter, which has OTX/CRX type binding sites and drives expression of five other transcripts. Band shift assays have shown that six of the seven candidate OTX/CRX elements bind CRX in vitro, possibly implying that the gene can regulate its own expression. These data may account for the differences in temporal expression IN VIVO: we have previously reported between these two species. |
