| First Author | Yanaka N | Year | 2000 |
| Journal | J Biol Chem | Volume | 275 |
| Issue | 20 | Pages | 14791-4 |
| PubMed ID | 10747865 | Mgi Jnum | J:62271 |
| Mgi Id | MGI:1858662 | Doi | 10.1074/jbc.C901047199 |
| Citation | Yanaka N, et al. (2000) Insertional mutation of the murine kisimo locus caused a defect in spermatogenesis. J Biol Chem 275(20):14791-4 |
| abstractText | Spermatogenesis is a developmental process that occurs in several phases and is regulated by a large number of gene products. An insertional transgenic mouse mutant (termed kisimo mouse) has been isolated that results in abnormal germ-cell development, showing abnormal elongated spermatids in the lumina of seminiferous tubules. We cloned the disrupted locus of kisimo and identified a novel testis-specific gene, THEG, which is specifically expressed in spermatids and was disrupted in the transgenic mouse. The yeast two-hybrid screening method revealed that THEG protein strongly interacts with chaperonin containing t-complex polypeptide-1epsilon, suggesting that THEG protein functions as a regulatory factor in protein assembly. Our findings indicate that the kisimo locus is essential for the maintenance of spermiogenesis and that a gene expression disorder may be involved in male infertility. |
