Primary Identifier | MGI:7467066 | Allele Type | Endonuclease-mediated |
Attribute String | No functional change, Recombinase, Reporter | Gene | Prss29 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | true | Is Wild Type | false |
molecularNote | Using CRISPR technology, IRES-CreEGFP-pA elements followed by a neo-selection cassette were inserted after the STOP codon in exon 6 of the Prss29 gene in the Embryonic Stem (ES) cells. Successfully targeted ES cells were used to generate chimeric mice by blastocyst injection for germline transmission. |