| First Author | Mizoguchi E | Year | 2000 |
| Journal | Int Immunol | Volume | 12 |
| Issue | 5 | Pages | 597-605 |
| PubMed ID | 10784605 | Mgi Jnum | J:110524 |
| Mgi Id | MGI:3640446 | Doi | 10.1093/intimm/12.5.597 |
| Citation | Mizoguchi E, et al. (2000) Regulatory role of mature B cells in a murine model of inflammatory bowel disease. Int Immunol 12(5):597-605 |
| abstractText | The spontaneous chronic colitis in TCR alpha mutant (TCRalpha(-/-)) mice mediated by CD4(+) TCRalpha(-)beta(+) T cells is more severe in the absence of mature B cells, suggesting a suppressive role of B cells and Ig in the development of chronic colitis. To investigate the direct role of B cells in the suppression of this colitis, cell transfer studies were performed in TCRalpha(-/-) x Igmu(-/-) (alphamu(-/-)) double-knockout mice. The chronic colitis was markedly attenuated in alphamu(-/-) mice after the adoptive transfer of peripheral B cells from TCRalpha(-/-) mice into 3- to 4-week-old alphamu(-/-) mice prior to the development of colitis. Furthermore, transfer of mature B cells from TCRalpha(-/-) mice markedly decreased the number of pathogenic colonic CD4(+) TCRalpha(-)beta(+) T cells in alphamu(-/-) mice with established colitis. This B cell effect required the presence of functional co-stimulatory molecules CD40 and B7-2 (CD86) but not B7-1 (CD80). These results indicate that mature B cells play an important role in the development of chronic colitis in TCRalpha(-/-) mice by directly regulating the pathogenic T cells (CD4(+) TCRalpha(-)beta(+) T cells). |
