| First Author | Takano H | Year | 2001 |
| Journal | Auton Neurosci | Volume | 87 |
| Issue | 2-3 | Pages | 249-57 |
| PubMed ID | 11476286 | Mgi Jnum | J:102795 |
| Mgi Id | MGI:3608078 | Doi | 10.1016/S1566-0702(00)00286-1 |
| Citation | Takano H, et al. (2001) Mechanical responses evoked by nerve stimulation in gastric muscles of mouse lacking inositol trisphosphate receptor. Auton Neurosci 87(2-3):249-57 |
| abstractText | Alteration of mechanical responses elicited by transmural nerve stimulation (TNS) was investigated in pylorus muscle of stomach isolated from mutant mice lacking expression of IP, type-1 receptor. In wild and mutant mice. TNS inhibited spontaneous contractions and generated an off-response at the cessation. The effects of inhibitors of neurotransmission revealed that in wild mice, acetylcholine and nitric oxide were involved as excitatory and inhibitory mediators, respectively. In mutant mice, a lack of nitroxidergic component with associated attenuation of cholinergic transmission was found. The off-response was inhibited by apamin in both mice. In mutant mice, spantide-sensitive excitatory response appeared in the presence of apamin. Acetylcholine and substance P enhanced while noradrenaline and sodium nitroprusside inhibited spontaneous contractions, in both wild and mutant mice; the actions were weaker in mutant mice than in wild mice for any agonists. The results indicate that pylorus smooth muscles receive cholinergic excitatory and nitroxidergic and non-adrenergic non-cholinergic inhibitory projections, and a lack of IP, type-1 receptor results in an impairment of cholinergic and nitroxidergic components, with no alteration of non-adrenergic non-cholinergic inhibitory projections. In addition, the mutation induces a substance P projection which is not detected in wild mice. |
