| First Author | Hahnel AC | Year | 1990 |
| Journal | Development | Volume | 110 |
| Issue | 2 | Pages | 555-64 |
| PubMed ID | 2133555 | Mgi Jnum | J:11608 |
| Mgi Id | MGI:60012 | Doi | 10.1242/dev.110.2.555 |
| Citation | Hahnel AC, et al. (1990) Two alkaline phosphatase genes are expressed during early development in the mouse embryo. Development 110(2):555-64 |
| abstractText | Alkaline phosphatase (AP) activity is stage specific in mouse embryos and may be associated with compaction and separation of trophectoderm from inner cell mass in preimplantation development. We previously sequenced a cDNA and two mouse AP genes that could contribute to the AP activity in embryos. Oligonucleotide primers were constructed from the three sequences and used in the reverse transcription-polymerase chain reaction technique to establish that two of the three AP isozymes are transcribed during preimplantation development. The predominant transcript (E-AP) is from a gene highly homologous to the human tissue-specific APs, but different from the mouse intestinal AP. Tissue non-specific (TN) AP also is transcribed, but there is approximately 10 times less TN-AP than E-AP transcript. The TN-AP isozyme is the predominant transcript of 7 to 14 day embryos and primordial germ cells. A switch in predominance from E-AP to TN-AP must occur during early postimplantation development. This study establishes a framework for experiments to determine the functions of the two isozymes during preimplantation development. |
