| First Author | Ichimiya I | Year | 1989 |
| Journal | Acta Otolaryngol Suppl | Volume | 457 |
| Pages | 148-53 | PubMed ID | 2784614 |
| Mgi Jnum | J:27663 | Mgi Id | MGI:75152 |
| Doi | 10.3109/00016488809138899 | Citation | Ichimiya I, et al. (1989) Experimental otitis media in mice. Immunohistochemical observations. Acta Otolaryngol Suppl (Stockh) 457:148-53 |
| abstractText | In order to identify T cells and B cells in the middle ear (ME) mucosa, immunohistochemical observations were performed on mice which were induced with immune-mediated otitis media with effusion (OME). C3H/HeN mice were challenged with dinitrophenylated ovalbumin in the ME, 2 weeks after the systemic immunization. At 1, 3 and 7 days post challenge, the mice were sacrificed, and IgG-, IgA- or IgM-positive cells, as well as Lyt-1- or Lyt-2-positive cells, were observed in the subepithelial layer of ME. IgG- or IgA-positive lymphocytes were detected at 7 days post challenge, whereas IgM-positive cells were rarely seen. At 3 days post challenge, the number of Lyt-1-positive lymphocytes exceeded that of Lyt-2-positive lymphocytes; however, at 7 days post challenge they were similar. Previous studies have suggested that immune complexes are a key factor in the generation of immune-mediated OME. However, it seems possible that delayed type hypersensitivity may contribute to the pathogenic mechanism of OME. |
