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Allele : Lcp2<tm2.1Mal> lymphocyte cytosolic protein 2; targeted mutation 2.1, Bernard Malissen

Primary Identifier  MGI:5617470 Allele Type  Targeted
Attribute String  Epitope tag, No functional change Gene  Lcp2
Transmission  Germline Strain of Origin  C57BL/6N
Is Recombinase  false Is Wild Type  false
molecularNote  Sequence for a Gly-Ser-Gly spacer, a One‐STrEP‐tag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site, was inserted in-frame at the 3′ end of the gene coding sequence in exon 21. Cre-mediated self-excision removed the cre+neo cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification. The presence of this tag does not affect protein expression.
  • mutations:
  • Insertion
  • synonyms:
  • Slp76<OST>,
  • SLP-76<OST>,
  • Slp76<OST>,
  • SLP-76<OST>,
  • Lcp2<tm2Mal>,
  • Lcp2<tm2Mal>
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

4 Publication categories

Trail: Allele