| First Author | Wang XY | Year | 1995 |
| Journal | J Biol Chem | Volume | 270 |
| Issue | 47 | Pages | 27999-8002 |
| PubMed ID | 7499280 | Mgi Jnum | J:29835 |
| Mgi Id | MGI:77359 | Doi | 10.1074/jbc.270.47.27999 |
| Citation | Wang XY, et al. (1995) Interleukin-11 induces complex formation of Grb2, Fyn, and JAK2 in 3T3L1 cells. J Biol Chem 270(47):27999-8002 |
| abstractText | Previous studies suggested that interleukin-11 (IL-11) induces the activation of mitogen-activated protein kinase (MAPK) in mouse 3T3L1 cells. However, the mechanisms by which IL-11 activates MAPK remain elusive. Our present results show that IL-11 promotes the formation of the active GTP-bound form of Ras, suggesting that IL-11 actions may be transduced in part through the Ras/MAPK signaling pathway. By immunoblotting and immunoprecipitation, we further demonstrate the association of tyrosine phosphoproteins with Grb2, an adaptor protein serving as a key intermediate for Ras activation. These phosphotyrosine-containing proteins have been subsequently identified to be JAK2, Fyn, and Syp. JAK2 and Fyn are transiently associated with Grb2 upon stimulation with IL-11, suggesting that JAK2 and Fyn may be involved in transducing signals from the IL-11 receptor-glycoprotein 130 to the Ras system through Grb2. Taken together, these results suggest that IL-11-induced interactions of JAK2, Fyn, and Grb2 may not only provide a novel mechanism for the activation of the Ras/MAPK system but also indicate cross-talk among diverse signaling pathways. |
